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1 ACADEMIC CURRICULUM VITAE Humberto De Vitto, Ph.D. The undersigned DE VITTO HUMBERTO, place of birth São Jose dos Campos (São Paulo) Brazil, date of birth 10/20 th /1982, and residing at São Jose dos Campos (São Paulo) Brazil, boulevard Heitor Vila Lobos n. 620 ZIP Alternative address: Piazza della Scienza, 2, Milan (MI), Italy Fiscal Code: DVTH BR 82 R20 Z 602 N Citizenship: Brazilian Mobile phone n :+39 (377) vitto@bioqmed.ufrj.br QUALIFICATION - DEGREES EARNED: August 2002 August 2006: Undergraduate in Biological Sciences at the Federal University of Fluminense, Niteroi, Rio de Janeiro, Brazil. Experimental monograph developed at the Center of Health and Science, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil, under advisor of Tenure Prof. Franklin D. Rumjanek (Ph.D.). *Degree final mark: 91/110 (that means 7.8/10.0 in Brazil). July 19 th _2006: Undergraduate degree. Title: Analysis of Y-chromosome haplotypes database frequency * As the following formula approved by the art. 10 in the Student Regulation ("Regolamento degli studenti",art.10: May 2007 October 2008: Graduate in Biological Sciences (Biological Chemistry) at the Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. Experimental Masters Thesis (MSc) developed at the Center of Health and Science, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil, under advisor of Tenure Prof. Franklin D. Rumjanek (Ph.D.). October 14 th 2008: Masters degree. Title: " Characterization of Y-chromosomal short tandem repeats (Y-STRs) mutations in a Rio de Janeiro population: implications for paternity testing and forensic analysis. October 2008 May 2013: Graduate in Biological Chemistry at the Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. Experimental Ph.D. Thesis (Ph.D) developed at the Center of Health and Science, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil, under advisor of Tenure Prof. Franklin D. Rumjanek (Ph.D.) and with collaborative period (May 2011 September 2011) at University of Illinois at Chicago, Chicago, (IL), USA, under advisor of Tenure Prof. James A. Radosevich (Ph.D.). May 07 th 2013: Philosophical Doctor degree. Title: Characterization of a Mechanism Linking Nitric Oxide Exposure that Induces Tumor Progression in Human Breast Cancer.
2 January 2014 December 2015: Postdoctoral Internship award at University of Milano- Bicocca, Milan, Italy. Research project developed at the Department of Biotechnology and Biosciences, under advisor of Dr. Ferdinando Chiaradonna (Ph.D.) December 10 th 2015: Postdoctoral. Title: Characterization of cancer metabolism induced by nutritional stress ACADEMIC APPOINTMENTS: January 2014 December 2015 March 2011 September 2011 April 2009 (credit hours: 20h) March 2009 (credit hours: 55h) Staff Research Scientist, University of Milano-Bicocca, Department of Biotechnology and Bioscience Dr. Ferdinando Chiaradonna Staff Research Scientist, University of Illinois at Chicago, CMBOD, College of Dentistry Dr. James A. Radosevich Research assistant, Applied Biosystems of Brazil Ltda (Sequencing of DNA in the platform ABI3130) Dr. Patricia Munerato Research assistant, Applied Biosystems of Brazil Ltda (Human Identification in ABI 3130) Dr. Selma Andrea Cavalli AWARDS: 1. The ISOBM 2011: Young Investigators Merit Awards at International Society of Oncology and BioMarkers, The Science without Borders: Post doctoral Internship from CAPES Brazil, BOOK CHAPTER: 1. Humberto De Vitto ; Antonio Galina.. Role of Mitochondria in Head & Neck Cancer. In: James A. Radosevich. (Org.). Head & Neck Cancer: Current Perspectives, Advances, and Challenges. 1 ed.: Springer, 2013, v. 1, p
3 2. Humberto De Vitto. Role of Hypoxia and Oxidative Stress in Mutagenesis and Metabolic Reprogramming: Driving Forces which Induce Malignancy as UV-Radiation Resistance. In: James A. Radosevich. (Org.). UV Radiation: Applications, Effects, and Properties. 1 ed.new York: Nova Science Publishers Inc, 2014, v. 1, p Humberto De Vitto ; Roberta Palorini ; Giuseppina Votta ; Ferdinando Chiaradonna. Mitochondria on Focus: Targeting the Cell Death Mechanism. In: James A. Radosevich. (Org.). Apoptosis and Beyond: The Many Ways Cells Die 1 ed.: Springer. In press 4. Humberto De Vitto ; Juan P. Valencia ; James A. Radosevich. General view of the cytoplasmic and nuclear features of apoptosis. In: James A. Radosevich. (Org.). Apoptosis and Beyond: The Many Ways Cells Die 1 ed.: Springer. In press PUBLICATION: 1. Abdullah Onul ; Kim Elseth ; Humberto De Vitto ; William Paradise ; Benjamin J. Vesper ; Gabor Tarjan ; G. Kenneth Haines III ; Franklin D. Rumjanek ; James A. Radosevich. Long-Term Adaptation of the Human Lung Tumor Cell Line A549 to Increasing Concentrations of Hydrogen Peroxide. Tumor Biology, v. 33, p , Gilson Costa dos Santos Junior ; Andréa Carla de Souza Góes ; Humberto De Vitto ; Carla Cristina Moreira ; Elizabeth Avvad ; Franklin D. Rumjanek ; Claudia Vitoria de Moura Gallo. Genomic instability at 13q31 locus and somatic mtdna mutation in D- loop site correlate with tumor aggressiveness in sporadic Brazilian breast cancer cases. Clinics (USP. Impress), v. 67, p , Benjamin J. Vesper ; Abdullah Onul ; G. Kenneth Haines III ; Gabor Tarjan ; Jiaping Xue ; Kim Elseth ; Bulent Aydogan ; Michael B Altman1 ; John C Roeske ; William Paradise ; Humberto De Vitto ; James A. Radosevich. Part I. Molecular and cellular characterization of high nitric oxide-adapted human breast adenocarcinoma cell lines. Tumor Biology, v. 34, p , Humberto De Vitto ; Bruna dos Santos Mendonça ; Kim Elseth ; Benjamin J. Vesper ; Elizabeth Avvad Portari ;Claudia Vitoria de Moura Gallo; William Paradise ; Franklin D. Rumjanek ; James A. Radosevich. Part II. Mitochondrial mutational status of high nitric oxide adapted cell line BT-20 (BT-20-HNO) as it relates to human primary breast tumors. Tumor Biology, v. 34, p , Humberto De Vitto ; Bruna dos Santos Mendonça ; Kim Elseth ; Abdullah Onul ; Jiaping Xue ; Benjamin J. Vesper ; Claudia Vitoria de Moura Gallo ; Franklin D. Rumjanek ; William Paradise ; James A. Radosevich. Part III. Molecular changes induced by high nitric oxide adaptation in human breast cancer cell line BT-20 (BT-20-
4 HNO): a switch from aerobic to anaerobic metabolism. Tumor Biology, v. 34, p , Humberto De Vitto ; Juan P. Valencia ; James A. Radosevich. Glutamine at Focus: Versatile Roles in Cancer. Tumor Biology, v, p 1-18, 2015 DOI /s Roberta Palorini ; Giuseppina Votta ; Yuri Pirola ; Sara De Palma ; Cristina Airoldi ; Humberto de Vitto ; Michele Vasso ; Francesca Ricciardiello ; Claudia Cirulli ; Raffaella Rizzi ; Francesco Nicotra ; Cecilia Gelfi ; Lilia Alberghina ; Ferdinando Chiaradonna. Protein Kinase A Activation Promotes Cancer Cell Resistance to Glucose Starvation and to Anoikis. Plos Genetics-D (submitted) SUMMARY PUBLISHED IN PROCEEDINGS OF CONFERENCES: 1. PALORINI, R. ; VOTTA, G. ; H. De Vitto ; PIROLA, Y. ; PALMA, S. ; AIROLDI, C. ; VASSO, M. ; RICCIARDIELLO, F. ; CIRULLI, C. ; RIZZI, R. ; NICOTRA, F. ; GELFI, C. ; ALBERGHINA, L. ; CHIARADONNA, F. 2015, Cambridge. Cancer and Metabolism United Kingdom : Abcam, v. 1. p VOTTA, G. ; PALORINI, R. ; H. De Vitto ; LOMBARDI, P. ; RICCIARDIELLO, F. ; ALBERGHINA, L. ; CHIARADONNA, F. 2015, Cambridge. Cancer and Metabolism United Kingdom : abcam, v. 1. p PALORINI, R. ; VOTTA, G. ; Humberto De Vitto ; RICCIARDIELLO, F. ; HILLER, K. ; CHIARADONNA, F. 2015, Leuven. Metabolism in Cancer and Stromal Cells. Gent : VIB Headquarters, v. 1. p CHIARADONNA, F. ; CIRULLI, C. ; Humberto De Vitto ; PALORINI, R. ; RICCIARDIELLO, F. ; VOTTA, G. Glucose metabolism as a tool for discovering cancer therapeutic targets. Milan v. 1. p VERMA, R. ; KIM, K. ; Kim Elseth ; AQIL, M. ; H. De Vitto ; James A. Radosevich. 2013, Chicago. 26th Annual Clinic and Research Day 2013, v. 1. p Z Deliu ; Kim Elseth ; AQIL, M. ; Abdullah Onul ; H. De Vitto ; James A. Radosevich. 2013, Chicago. 26th Annual Clinic and Research Day 2013, v. 1. p DOS SANTOS, R. S ; Humberto De Vitto ; AMOEDO, N. D ; DA-SILVA, W.S. 2011, Foz do Iguaçu. XXXIX Annual Meeting of the Brazilian Biochemistry and Molecular Biology Society (SBBq), 2011
5 8. Humberto De Vitto ; MENDONÇA, B.S. ; Kim Elseth ; Abdullah Onul ; NOWEDER, ; J Xue ; Benjamin J. Vesper ; MOURA-GALLO, C.V. ; Rumjanek, F. D. ; James A. Radosevich. 2011, Florence. The 39th Meeting of the International Society og Oncology and BioMarkers, v. 32. p Humberto De Vitto ; MENDONÇA, B.S. ; Kim Elseth ; Abdullah Onul ; Benjamin J. Vesper ; PORTARI, E. A ; MOURA-GALLO, C.V. ; James A. Radosevich ; Franklin D. Rumjanek. 2011, Florence. The 39th Meeting of the International Society of Oncology and BioMarkers, v. 32. p Abdullah Onul ; H. De Vitto ; Kim Elseth ; Benjamin J. Vesper ; Z Deliu ; Rumjanek, F. D. ; James A. Radosevich. 2011, Florence. the 39th Meeting of the International Society of Oncology and BioMarkers., v. 1. p Humberto De Vitto ; CORDEIRO, C.R.S. ; MENDONÇA, B.S. ; CALDEIRA, C. M. ; PAGNONCELLI, D. ; MOURA-GALLO, C.V. ; RUMJANEK, F. D. 2010, Foz do Iguaçu. XXXIX Annual Meeting of SBBq, MENDONÇA, B.S. ; Humberto De Vitto ; PORTARI, E. A ; MOURA-GALLO, C.V. ; RUMJANEK, F. D. 2010, Rio de Janeiro. XXXII Jornada Giulio Massarani de Iniciação Científica, Artística e Cultural, Humberto De Vitto ; VOLKWEIS, R. O. ; CALDEIRA, C. M. ; RUMJANEK, F. D., 2008, Salvador-BA. SBG/Sociedade Brasileira de Genética. LIV Reunião Anual, Reunião Anual Programa e Índices, 2008., 2008, v. LIV. PRESENTATION OF WORK: 1. Humberto De Vitto ; MENDONÇA, B.S. ; Kim Elseth ; Abdullah Onul ; NOWEDER, ; J Xue ; Benjamin J. Vesper ; MOURA-GALLO, C.V. ; Rumjanek, F. D. ; James A. Radosevich (Presentation/Congress) RESEARCH TECHNIQUES EXPERTISE: 1. DNA, RNA and protein extraction 2. DNA sequencing techniques; 3. quantitative-pcr (q-pcr) and traditional-pcr; 4. Cell culture (human cell lines, as pancreatic cancer cell lines, and murine cell lines); 5. Proliferation and survival assays; 6. Clonogenic assay; 7. Mitochondrial respiration analysis; 8. FACS analysis; 9. Western Blot analysis;
6 10. Metabolites quantification analysis; 11. Enzymatic measurement assays; 12. Fluorescence and immunohistochemistry microscopy LANGUAGE SKILLS: Portuguese: English: mother tongue, fluent understand: good; speak: good; read: good and write: good REFERENCES: James. A. Radosevich, Ph.D. Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, 801 S. Paulina St., Chicago, IL 60612, USA Benjamin. J. Vesper, Ph.D. Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, 801 S. Paulina St., Chicago, IL 60612, USA Ferdinando Chiaradonna, Ph.D. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, Milan, MI 20126, Italy.
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