Norbert W. Seidler, Ph.D.

Transcription

1 Curriculum Vitae Norbert W. Seidler, Ph.D. Professor and Chairman Department of Biochemistry Kansas City University of Medicine and Biosciences 1750 Independence Avenue Kansas City, Missouri (office) (fax) Education Bachelor s Degree in Psychology (June 1981) Mentor: Darcy B. Kelley, Ph.D. (senior thesis: Androgen-regulated auditory system of X. laevis) Princeton University Princeton, New Jersey Doctorate Degree in Biochemistry (May 1988) Mentor: Norbert I. Swislocki, Ph.D. (thesis: Ca 2+ - regulation and cell aging) University of Medicine and Dentistry of New Jersey - Graduate School of Biomedical Sciences Newark, New Jersey Postdoctoral Training April 1988 to July 1991 Postdoctoral Research Associate Studies involved the sarcoplasmic reticulum Ca 2+ -ATPase and related ATPases Mentor: Anthony N. Martonosi, M.D. Department of Biochemistry and Molecular Biology State University of New York - Health Science Center at Syracuse Syracuse, New York Current Faculty Appointments Kansas City University of Medicine and Biosciences Department of Biochemistry July 2004 to the Present: Department Chair March 2000 to the Present: Professor of Biochemistry University of Missouri - Kansas City School of Medicine Department of Anesthesiology February 2006 to the Present: Adjunct Professor of Anesthesiology Research 1

2 Previous Faculty Appointments Kansas City University of Medicine and Biosciences Department of Biochemistry July 1995 to March 2000: Associate Professor of Biochemistry September 1991 to June 1995: Assistant Professor of Biochemistry Research Publications [1] Freund TS, Hermann G, Hayes R, Seidler N: High affinity calcium binding proteins in human saliva. In Calcium Binding Proteins to Health and Disease (B. de Bernard et al., eds) 1983;71-72, Amsterdam: Elsevier Press. [2] Lea MA, Grasso SV, Hu J, Seidler N: Factors affecting the assay of histone H1 and polylysine by binding of Coomassie Blue G. Anal Biochem 1984;141: [3] Bennun A, Seidler NW, DeBari VA: Divalent metals in the regulation of hemoglobin affinity for oxygen. Ann NY Acad Sci 1985;463: [4] Bennun A, Seidler NW, DeBari VA: A model for the regulation of hemoglobin affinity for oxygen. Biochem Soc Trans 1985;13: [5] Seidler NW, Jona I, Vegh M, Martonosi A: Cyclopiazonic acid is a specific inhibitor of the Ca 2+ ATPase of sarcoplasmic reticulum. J Biol Chem 1989;264: [6] Molnar E, Seidler NW, Jona I, Martonosi A: The binding of monoclonal and polyclonal antibodies to the Ca 2+ ATPase of sarcoplasmic reticulum; effects on interactions between ATPase molecules. Biochim Biophys Acta 1990;1023: [7] Martonosi AN, Jona I, Molnar E, Seidler NW, Buchet R, Varga S: Emerging views on the structure and dynamics of the Ca 2+ ATPase in sarcoplasmic reticulum. FEBS Lett 1990;268: [8] Seidler NW, Swislocki NI: Ca 2+ transport activities of inside-out vesicles prepared from density-separated erythrocytes from rat and human. Molec Cell Biochem 1991;105: [9] Buchet R. Varga S, Seidler NW, Molnar E, Martonosi A: Polarized infrared attenuated total reflectance spectroscopy of the Ca 2+ -ATPase of sarcoplasmic reticulum. Biochim Biophys Acta 1991;1068: [10] Molnar E. Vara S, Jona I, Seidler NW, Martonosi A: Immunological relatedness of the sarcoplasmic reticulum Ca 2+ -ATPase and the Na+, K + ATPase. Biochim Biophys Acta 1992;1103: [11] Seidler NW, Swislocki NI: The effects of pentoxifylline on the plasma membrane Ca 2+ ATPase in age-separated rat and human erythrocytes. J Clin Pharmacol 1992;32: [12] Russo PJ, Phillips JW, Seidler NW: The role of lipid peroxidation in McArdle's Disease: applications for treatment of other myopathies. Med Hypotheses 1992;39: [13] Russo PJ, Seidler NW: NSAID-induced gastroenteropathy: a biochemical dissection. Hospital Practice 1992;27:

3 [14] Gatton-Umphress TL, Weber KA, Seidler NW: Methionine metabolism: a window on carcinogenesis? Hospital Practice 1993;28: [15] Rajguru SU, Yeargans GS and Seidler NW: Exercise causes oxidative damage to rat skeletal muscle microsomes while increasing cellular sulfhydryls. Life Sciences 1994;54: [16] Guichard PC, Burkhardt JE, Seidler NW: Diabetic cardiomyopathy:the significance of creatine. Med Hypotheses 1995;45: [17] Kraft A, Cassetta M, Seidler NW: Inhibition of the cardiac sarcoplasmic reticulum Ca 2+ - ATPase by glucose 6-phosphate is Ca 2+ dependent. Life Sciences 1998;62: [18] Fitzgerald C, Swearengin TA, Yeargans G, McWhorter D, Cucchetti B, Seidler NW: Nonenzymatic glycosylation (or glycation) and inhibition of the pig heart cytosolic aspartate aminotransferase by glyceraldehyde 3-phosphate. J Enzym Inhib 1999;15: [19] Swearengin TA, Fitzgerald C, Seidler NW: Carnosine prevents glyceraldehyde 3- phosphate-mediated inhibition of aspartate aminotransferase. Arch Toxicol 1999;73: [20] Seidler NW: Carnosine prevents the glycation-induced changes in electrophoretic mobility of aspartate aminotransferase. J Biochem Molec Toxicol 2000;14: [21] Seidler NW, Seibel I: Glycation of aspartate aminotransferase and conformational flexibility. Biochem Biophys Res Commun 2000;277: [22] Seidler NW, Shokry SS, Nauth J: Properties of a glycation product derived from carnosine. J Biochem Mol Biol Biophys 2001;5: [23] Seidler NW, Yeargans GS: Effects of thermal denaturation on protein glycation. Life Sci 2002;70: [24] Southwell JL, Yeargans GS, Kowalewski C, Seidler NW: Effects of acrolein on cytosolic aspartate aminotransferase. J Enz Inhib Med Chem 2002;17: [25] Seidler NW, Kowalewski C: Methylglyoxal-induced glycation affects protein topography. Arch Biochem Biophys 2003;410: [26] Yeargans GS, Seidler NW: Carnosine promotes the heat denaturation of glycated protein. Biochem Biophys Res Commun 2003;300: [27] Hobart LJ, Seibel I, Yeargans GS, Seidler NW: Anti-crosslinking properties of carnosine: significance of histidine. Life Sciences 2004;75: [28] Seidler NW, Yeargans GS, Morgan TG: Carnosine disaggregates glycated α-crystallin: an in vitro study. Arch Biochem Biophys 2004;427: [29] [COVER ARTICLE] Seidler NW, Yeargans GS: Albumin-bound polyacrolein: implications for Alzheimer s disease. Biochem Biophys Res Commun 2004;320: [30] Mehta, AD, Seidler NW: β-alanine suppresses heat inactivation of lactate dehydrogenase. J Enzyme Inhib Med Chem 2004; 20(2): [31] Seidler NW: Carbonyl-induced enzyme inhibition: mechanisms and new perspectives. Current Enzyme Inhibition 2005;1(1): [32] Seidler NW, Yeargans GS: Can histidines stabilize a carnosine-derived pyrazinium radical? Ann NY Acad Sci 2005;1043:888. 3

4 [33] Seidler NW, Squire TJ: Aβ-polyacrolein aggregates: novel mechanism of plastic formation in senile plaques. Biochem Biophys Res Commun 2005;335: [34] Seidler NW, Craig HD, Squire TJ: Endogenous plastic composite material in the Alzheimer s brain. Med Hypotheses 2006;67: [35] Swearengin TA, Fibuch EE, Seidler NW: Sevoflurane modulates the activity of glyceraldehyde 3-phosphate dehydrogenase. J Enzyme Inhib Med Chem 2006;21(5): [36] Seidler NW, Michelson RC: Polymer templates have an organizing effect on Aβ aggregation. In (AP Chan, editor) Alzheimer s Disease Research Trends, Nova Science Publishers, 2007, pp [37] Roberts RD, Fibuch EE, Heal ME, Seidler NW. Production of a novel neuromelanin at the sevoflurane-water interface. Biochem Biophys Res Commun 2007;363(1): [38] Willsie PS, Harris CK, Seidler NW. Cu(II)-Catalyzed Formation of Plastic Composites in AD. New Trends in Alzheimer and Parkinson Related Disorders: ADPD 2007 (Editors: Hanin I., Windisch M., Poewe W., Fisher A) Medimond International Proceedings, Bologna: Italy, 2008, pp [39] Craig HD, Eklund JD, Seidler NW. Trifluoroethanol increases albumin's susceptibility to chemical modification. Arch Biochem Biophys 2008;480(1): [40] Seidler NW, Eklund JD. Interfacial effects on the conformation of amyloid-beta peptide. Protein Pept Lett 2009;16(2): [41] Eklund JD, Seidler NW. Computational analysis of shifts in the fluorescence spectra of human serum albumin. In (HR Arabnia, MQ Yang, editors) Proceedings of the 2009 International Conference on Bioinformatics and Computational Biology, Volume I, CSREA Press, 2009, pp [42] Miller ED, Fibuch EE, Seidler NW. Toxicity of a serotonin-derived neuromelanin. Biochem Biophys Res Commun 2010; 391(2): [43] Pieters BJ, Fibuch EE, Eklund JD, Seidler NW. Inhaled anesthetics promote albumin dimerization through reciprocal exchange of subdomains. Biochemistry Research International, Volume 2010 (2010), Article ID , 7 pages, doi: /2010/ [44] Murphy MM, Miller ED, Fibuch EE, Seidler NW. Redox mechanism of neurotoxicity by a serotonin-acrolein polymeric melanoid. Neurotoxicity Res 2010;19(2): [45] Pattin AE, Ochs S, Theisen CS, Fibuch EE, Seidler NW. Isoflurane s effect on interfacial dynamics in GAPDH influences methylglyoxal reactivity. Arch Biochem Biophys 2010;498(1):7-12. [46] Theisen CS, Seidler NW. Computational Determination of Aqueous Concentrations of Inhalational Anesthetics. In (HR Arabnia, Q-N Tran, editors) Proceedings of the 2010 International Conference on Bioinformatics and Computational Biology, Volume II, CSREA Press, 2010, pp [47] Brownrigg TD, Theisen CS, Fibuch EE, Seidler NW. Carnosine protects against the neurotoxic effects of a serotonin-derived melanoid. Neurochem Res 2011;36(3): doi: /s

5 [48] McClintick CA, Theisen CS, Ferns JE, Fibuch EE, Seidler NW. Isoflurane preconditioning involves upregulation of molecular chaperone genes. Biochem Biophys Res Commun 2011;411(2): doi: /j.bbrc [49] Kim JY, Theisen CS, Seidler NW. GAPDH architecture at low guanidine concentrations: first derivative analysis of the descending slope of the UV absorbance peak. In (HR Arabnia, Q-N Tran, editors) Proceedings of the 2011 International Conference on Bioinformatics and Computational Biology, Volume II, CSREA Press, 2011, pp [50] Baker MR, Benton SK, Theisen CS, McClintick CA, Fibuch EE, Seidler NW Isoflurane s effect on protein conformation as a proposed mechanism for preconditioning. Biochemistry Res Int Volume 2011(2011), Article ID [51] Ferns JE, Theisen CS Fibuch EE, Seidler NW Protection against protein aggregation by alpha-crystallin as a mechanism of preconditioning. Neurochemical Research 2012;37(2): doi: /s [52] Seidler NW. Basic biology of GAPDH. Adv Exp Med Biol. 2013;985:1-36. [53] Seidler NW. GAPDH and intermediary metabolism. Adv Exp Med Biol. 2013;985: [54] Seidler NW. Compartmentation of GAPDH. Adv Exp Med Biol. 2013;985: [55] Seidler NW. Functional diversity. Adv Exp Med Biol. 2013;985: [56] Seidler NW. GAPDH, as a virulence factor. Adv Exp Med Biol. 2013;985: [57] Seidler NW. Target for diverse chemical modifications. Adv Exp Med Biol. 2013;985: [58] Seidler NW. Dynamic oligomeric properties. Adv Exp Med Biol. 2013;985: [59] Seidler NW. Multiple binding partners. Adv Exp Med Biol. 2013;985: [60] Seidler NW. GAPDH in Anesthesia. Adv Exp Med Biol. 2013;985: [61] Theisen CS, Seidler KA, Seidler NW. Computational criteria for the disablement of human GAPDH pseudogenes. In (HR Arabnia, Q-N Tran, editors) Proceedings of the 2012 International Conference on Bioinformatics and Computational Biology, CSREA Press, 2012, pp [62] Montalbano AJ, Theisen CS, Fibuch EE, Seidler NW. Isoflurane enhances the moonlighting activity of GAPDH: implications for GABA A receptor trafficking, ISRN Anesthesiology, Volume 2012 (2012), Article ID , 7 pages. [63] Mason SO, Theisen CS, Seidler NW. Neuronal protection by a variant of GAPDH pseudogene P44 in AD. Advances in Alzheimer s Disease 2012;1(3): [64] Gorshkalova OV, Seidler NW. Effects of chloride ions on GAPDH conformation. In (HR Arabnia, Q-N Tran, editors) Proceedings of the 2013 International Conference on Bioinformatics and Computational Biology, CSREA Press, 2013, pp

6 Research Abstracts/Presentations (From 1992 to the Present) [1] Effects of aging and exercise on protein sulfhydryls in various tissues of the rat. SU Rajguru, GS Yeargans, NW Seidler. Presented at the Inaugural Symposium on Molecular Mechanisms of Aging sponsored by SEP (Marion Merrill Dow Foundation, University of Kansas and University of Missouri-Kansas City), Kansas City, Missouri, October 15-18, [2] Effects of exercise on the phospholipid and malondialdehyde levels in cardiac and skeletal muscle sarcoplasmic reticulum from sedentary young and old rats. GS Yeargans, SU Rajguru, NW Seidler. Presented at the Inaugural Symposium on Molecular Mechanisms of Aging sponsored by SEP (Marion Merrill Dow Foundation, University of Kansas and University of Missouri-Kansas City), Kansas City, Missouri, October 15-18, [3] NSAID-induced gastroenteropathy: the role of glutathione-reactive leukotrienes. PJ Russo, NW Seidler. Presented at the AOA 36th Annual Research Conference, San Diego, California, November 1-4, Journal of the American Osteopathic Association (1992) 92: 1131, abstr [4] Protein crosslinking in skeletal muscle after exhaustive exercise of sedentary rats. GS Yeargans, SU Rajguru, NW Seidler. Presented at Experimental Biology 93 (FASEB Meeting), New Orleans, Louisiana, March 28-April 1, [5] The role of lipid peroxidation in McArdle s disease. PJ Russo, JW Phillips, NW Seidler. Presented at the First Annual UHS Research Symposium, Kansas City, Missouri, April 23, [6] NSAID-indused gastroenteropathy. PJ Russo, NW Seidler. Presented at the First Annual UHS Research Symposium, Kansas City, Missouri, April 23, [7] The pathogenesis of cancer: a look at methionine. TL Gatton-Umphress, NW Seidler. Presented at the First Annual UHS Research Symposium. Kansas City, Missouri, April 23, [8] The effects of exercise on various tissues. SU Rajguru, NW Seidler. Presented at the First Annual UHS Research Symposium, Kansas City, Missouri, April 23, [9] Oxidative damage in aging. G Yeargans, NW Seidler. Presented at the First Annual UHS Research Symposium, Kansas City, Missouri, April 23, [10] The role of environmentally-induced inactivation of antithrombin III in cerebrovascular insufficiency. KA Weber, NW Seidler. Presented at the AOA 37th Annual Research Conference Boston, Massachusetts, October 10-14, 1993, Journal of the American Osteopathic Association (1993) 93:867, abstr [11] Substance P and increased intestinal permeability in inflammatory bowel disease. PJ Russo, NW Seidler. Presented at the AOA 37th Annual Research Conference. Boston, Massachusetts, October 10-14, 1993, Journal of the American Osteopathic Association (1993) 93:867, abstr [12] Effects of cytoprotective amines on cardiac tissue. JE Burkhardt, F Farokhi, CA Sites, PC Guichard, NW Seidler. Presented at the AOA 37th Annual Research Conference, Boston, Massachusetts, October 10-14, 1993, Journal of the American Osteopathic Association (1993) 93:867, abstr [13] Methionine metabolism and carcinogenesis. TL Gatton-Umphress, KA Weber, NW Seidler. Presented at the 1st Annual Meeting of The Oxygen Society, Charleston, South Carolina, November 12-17, [14] Diabetic cardiomyopathy: the significance of creatine. P Guichard, NW Seidler. Presented at the Second Annual UHS Research Symposium, Kansas City, Missouri, April 1,

7 [15] Molecular modeling of antithrombin and its interactions. KA Weber, NW Seidler. Presented at the AOA 38th Annual Research Conference, San Fransico, California, November 13-17, 1994, Journal of the American Osteopathic Association (1994) 94:675, abstr [16] Effects of acetaldehyde on cardiac cytosolic proteins. PC Guichard, NW Seidler. Presented at the AOA 38th Annual Research Conference, San Fransico, California, November 13-17, 1994, Journal of the American Osteopathic Association (1994) 94:675, abstr [17] Chemical modification of cardiac cytosolic components by acetaldehyde. F Farokhi, NW Seidler. Presented at the American College of Physicians Annual Session, Atlanta, Georgia, March 17-19, [18] Decrease in amines in cardiac cytosols following incubation with acetaldehyde. F Farokhi, PC Guichard, NW Seidler. Presented at Experimental Biology 95(FASEB Meeting), Atlanta, Georgia, April 9-13, 1995, FASEB Journal (1995) 9:A895, abstr [19] Evidence for a carbohydrate-mediated inhibition of cardiac sarcoplasmic reticulum Ca2+- ATPase activity. NW Seidler, A Kraft, P Freeman. Presented at the 2nd Annual Meeting of The Oxygen Society, Pasadena, California, November 16-20, [20] A model implicating S-adenosylmethionine depletion as a causal factor in carcinogenesis. NW Seidler, WC Snider. Presented at the 2nd Annual Meeting of The Oxygen Society, Pasadena, California, November 16-20, [21] Evidence for a carbohydrate-mediated inhibition of cardiac sarcoplasmic reticulum Ca2+- ATPase activity. A Kraft, NW Seidler. Presented at the Fourth Annual UHS Research Symposium, Kansas City, Missouri, May 10, [22] A model implicating S-adenosylmethionine depletion as a causal factor in carcinogenesis. WC Snider, NW Seidler. Presented at the Fourth Annual UHS Research Symposium, Kansas City, Missouri, May 10, [23] Effects of glyceraldehyde 3-phosphate on rat cardiac microsomes. NW Seidler, M Cassetta. Presented at the 3rd Annual meeting of The Oxygen Society, Miami, Florida, November 21-25, [24] Reactivity of glyceraldehyde 3-phosphate on diverse amines. M Cassetta, L Smith, G Yeargans, NW Seidler. Presented at the Fifth Annual UHS Research Symposium, Kansas City, Missouri, May 9, [25] Glyceraldehye 3-phosphate reacts with non-protein amines and protein amino groups. M Cassetta, L Smith, G Yeargans, A Kraft, NW Seidler. Presented at the AOA 41st Annual Research Conference, San Antonio, Texas, October 19-23, 1997, Journal of the American Osteopathic Association (1997) 97:543, abstr. P49. [26] α, β, and γ-amino acids as model compounds for cardioprotective agents. NW Seidler. Presented at the UHS Seminar Series, University of Health Sciences, Kansas City, Missouri, November 7, [27] Aldehyde scavengers: a potentially new category of cardioprotective agents. NW Seidler. Presented at Cardiovascular Day V, University of Missouri- Columbia, Columbia, Missouri, February 9, [28] Analysis of rat cardiac microsomal proteins following incubation with calpain. B Cucchetti, NW Seidler. Presented at the Sixth Annual UHS Research Symposium, Kansas City, Missouri, February 27, [29] Changes in differential centrifugation affect the yield and purity of cardiac transverse tubules. C Fitzgerald, T. Swearengin, NW Seidler. Presented at the Sixth Annual UHS Research Symposium, Kansas City, Missouri, February 27,

8 [30] Proteolysis of the cardiac sarcoplasmic reticulum Ca 2+ -ATPase by calpain. NW Seidler, B Cucchetti. Presented at the 42nd Annual Meeting of the Biophysical Society, Kansas City, Missouri, February 22-26, 1998, Published in Biophysical Journal (1998) 74:A360. [31] Inhibition of porcine heart cytosolic aspartate transaminase by glyceraldehyde 3-phosphate. C Fitzgerald, T Swearengin, D McWhorter, G. Yeargans, B Cucchetti, NW Seidler. Presented at the 42nd Annual AOA Research Conference, New Orleans, Louisiana, October 5-9, 1998, Journal of the American Osteopathic Association (1998) 98:454, abstr. B21. [32] Modulation of the inhibitory effects of glyceraldehyde 3-phosphate on cardiac aspartate aminotransferase. T. Swearengin, C Fitzgerald, G Yeargans, D McWhorter, S Shokry, NW Seidler. Presented at the Seventh Annual UHS Research Symposium, Kansas City, Missouri, January 15, [33] Carnosine has a high affinity to cardiac aspartate aminotransferase. G Yeargans, NW Seidler. Presented at the 1st Annual Southwest Missouri Collegiate Chemistry Symposium, Southwest Missouri State University, Springfield, Missouri, March 27, [34] Carnosine prevents glycreraldehye 3-phosphate mediated inhibition of the cardiac asparate aminotransferase. NW Seidler, TA Swearengin, GS Yeargans. Presented at the 16 th American Peptide Symposium, Minneapolis, Minnesota, June 26 - July 1, [35] Carnosine preserves protein structure. T. Swearengin, NW Seidler. Presented at the 43 rd Annual AOA Research Conference, San Francisco, California, October 24-28, 1999, Journal of the American Osteopathic Association (1999) 99:483, abstr. B18. [36] L-Carnosine (β-alanyl-l-histidine) and L-histidine but not β-alanine prevent protein glycation. NW Seidler, SS Shokry, J Nauth. Presented at the 6th Annual Meeting of The Oxygen Society, New Orleans, Louisiana, November 21-25, 1999, Free Radical Biology and Medicine (1999) 27:S98, abstr [37] Aspartate aminotransferase as a model system for studying glycation reactions: implications for ageing, atherosclerosis and Alzheimer s disease. NW Seidler. Pharma- Transfer (Online Publishing Group), Ballantyne Ross, Ltd., April 4, [38] Glycation in skeletal muscle: a comparison of extensor digitorum longus and soleus muscles from swim exercised rats. DL McWhorter, NW Seidler, GS Yeargans. Presented at Experimental Biology 2000, San Diego, California, April15-18, 2000, FASEB Journal (2000) 14:A620, abstr [39] Glycation-induced modification of L-carnosine. SS Shokry, NW Seidler. Presented at the Eighth Annual UHS Research Symposium, Kansas City, Missouri, April 28, [40] Appearance of a fluorescent glycation product following incubation of L-carnosine with glyceraldehyde 3-phosphate. J Nauth, NW Seidler. Presented at the Eighth Annual UHS Research Symposium, Kansas City, Missouri, April 28, [41] Aspartate aminotransferase interactions with carnosine. GS Yeargans, NW Seidler. Presented at the 44 th Annual AOA Research Conference, Orlando, Florida, October 29- November 1, Journal of the American Osteopathic Association (2000) 100:583, abstr. B17. [42] Absorbance properties of a carnosine-containing glycation product. SS Shokry, NW Seidler. Presented at the 44 th Annual AOA Research Conference, Orlando, Florida, October 29-November 1, Journal of the American Osteopathic Association (2000) 100:585, abstr. B23. [43] A fluorescent glycation product derived from carnosine. J Nauth, SS Shokry, NW Seidler. Presented at the 44 th Annual AOA Research Conference, Orlando, Florida, October 29-8

9 November 1, Journal of the American Osteopathic Association (2000) 100:583, abstr. B16. [44] Effects of protein glycation on 1-anilinonaphthalene 8-sulphonate binding. NW Seidler, I Seibel. Presented at the 7 th Annual Meeting of the Oxygen Society, San Diego, California, November 16-20, 2000, Free Radical Biology and Medicine (2000) 29:S85, abstr [45] Association between acrolein and hydrogen peroxide on protein modification. JL Southwell, NW Seidler. Presented at the Ninth Annual UHS Research Symposium, Kansas City, Missouri, April 13, [46] Lipoxidation of cytosolic aspartate aminotransferase. JL Southwell, NW Seidler. Presented at the 45 th Annual AOA Research Conference, San Diego, California, October 20-24, Journal of the American Osteopathic Association (2001) 101:550, abstr. B18. [47] Methylglyoxal-induced changes in protein conformation. C Kowalewski, NW Seidler. Presented at the Tenth Annual UHS Research Symposium, Kansas City, Missouri, January 18, [48] L-Carnosine: an anti-ageing nutrient. NW Seidler. Presented at the UHS Grand Rounds seminar series, Kansas City, Missouri, April 17, [49] Effects of methylglyoxal on the conformation of aspartate aminotransferase. C Kowalewski, NW Seidler. Presented at the 46 th Annual AOA Research Conference, Las Vegas, Nevada, October 7-11, Journal of the American Osteopathic Association (2002) 102:502, abstr. B01. [50] Carnosine promotes thermal instability of glycated proteins. GS Yeargans, NW Seidler. Presented at the 46 th Annual AOA Research Conference, Las Vegas, Nevada, October 7-11, Journal of the American Osteopathic Association (2002) 102:505, abstr. B13. [51] Carnosine: properties of a neuroprotective agent. L Hobart, NW Seidler. Presented at the Eleventh Annual UHS Research Symposium, Kansas City, Missouri, January 17, [52] Carnosine: thermal unfolding of glycated protein. NW Seidler. Presented at the Eleventh Annual UHS Research Symposium, Kansas City, Missouri, January 17, [53] De-aggregation of glycated α-crystallin by carnosine. TG Morgan, GS Yeargans, NW Seidler. Presented at the 47 th Annual AOA Research Conference, New Orleans, Louisiana, October 12-16, Journal of the American Osteopathic Association (2003) 103:442, abstr. B06. [54] Anti-crosslinking activity of anserine. LJ Hobart, NW Seidler. Presented at the 47 th Annual AOA Research Conference, New Orleans, Louisiana, October 12-16, Journal of the American Osteopathic Association (2003) 103:443, abstr. B07. [55] The scorpion model: carnosine stabilizes a glycation-derived pyrazinium radical. NW Seidler, GS Yeargans. Presented at the Twelfth Annual UHS Research Symposium, Kansas City, Missouri, January 16, [56] Carnosine disaggregates glycated α-crystallin. Morgan TG, Yeargans GS, NW Seidler. Presented at the Twelfth Annual UHS Research Symposium, Kansas City, Missouri, January 16, [57] Can histidines stabilize a carnosine-derived pyrazinium radical? NW Seidler, GS Yeargans. Presented at the 8th International Symposium on the Maillard Reaction, Charleston, South Carolina, August 28, [58] β-alanine suppresses heat inactivation of lactate dehydrogenase. AD Mehta, NW Seidler. Presented at the 3rd Annual AMA-MSS Research Poster Session, Atlanta, Georgia, December 4, [59] β-alanine reactivates thermally denatured lactate dehydrogenase. AD Mehta, NW Seidler. Presented at the Thirteenth Annual KCUMB Research Symposium, Kansas City, Missouri, February 25,

10 [60] Proposed mechanism for Alzheimer s disease: endogenous formation of plastic. NW Seidler, GS Yeargans. Presented at the Thirteenth Annual KCUMB Research Symposium, Kansas City, Missouri, February 25, [61] Sevroflurane modulates the activity of glyceraldehyde 3-phosphate dehydrogenase. TA Swearengin, EE Fibuch, NW Seidler. Presented at Midwest Anesthesia Residents Conference 2005, Madison, Wisconsin, April 1, [62] Sevroflurane inhibits oligomerization of human serum albumin. BJ Pieters, EE Fibuch, NW Seidler. Presented at Midwest Anesthesia Residents Conference 2005, Madison, Wisconsin, April 1, [63] Sevroflurane modulates the activity of glyceraldehyde 3-phosphate dehydrogenase. TA Swearengin, EE Fibuch, NW Seidler. Presented at KCALSI Research Day, Kansas City, Missouri, April 6, [64] Sevroflurane inhibits oligomerization of human serum albumin. BJ Pieters, EE Fibuch, NW Seidler. Presented at KCALSI Research Day, Kansas City, Missouri, April 6, [65] Aβ-polyacrolein aggregates: novel mechanism of plastic formation in senile plaques. NW Seidler, TJ Squire. Presented at the Fourteen Annual KCUMB Research Symposium, Kansas City, Missouri, February 17, [66] Tyrosine polarization as a method for measuring amyloid-β aggregation. KK Brott, NW Seidler. Presented at the Fourteen Annual KCUMB Research Symposium, Kansas City, Missouri, February 17, [67] Sevroflurane modulates the activity of glyceraldehyde 3-phosphate dehydrogenase. AE Pattin, EE Fibuch, NW Seidler. Presented at Midwest Anesthesia Residents Conference 2006, Chicago, Illinois, March 17, [68] Isoflurane affects the molecular conformation of GAPDH. S Ochs, EE Fibuch, NW Seidler. Presented at Midwest Anesthesia Residents Conference 2006, Chicago, Illinois, March 17, [69] Sevroflurane modulates the activity of glyceraldehyde 3-phosphate dehydrogenase. AE Pattin, EE Fibuch, NW Seidler. Presented at KCALSI Research Day, Kansas City, Missouri, March 30, [70] Isoflurane affects the molecular conformation of GAPDH. S Ochs, EE Fibuch, NW Seidler. Presented at KCALSI Research Day, Kansas City, Missouri, March 30, [71] Aβ Fibril-promoting agents. H Craig, KK Brott, NW Seidler. Presented at the 50 th Annual AOA Research Conference, Las Vegas, Nevada, October 15-18, Journal of the American Osteopathic Association (2006) 106:495, abstr. B01. [72] Plastic fragments of Aβ-polyacrolein in the Alzheimer s brain. TJ Squire, HD Craig, NW Seidler. Presented at the 50 th Annual AOA Research Conference, Las Vegas, Nevada, October 15-18, Journal of the American Osteopathic Association (2006) 106:496, abstr. B04. [73] Effects of divalent cations on the production of polyacrolein-aβ. CK Harris, NW Seidler. Presented at the Fifteen Annual KCUMB Research Symposium, Kansas City, Missouri, February 9, [74] Promotion of lipoxidation of human serum albumin by solvent hydrophobicty. HD Craig, NW Seidler. Presented at the Fifteen Annual KCUMB Research Symposium, Kansas City, Missouri, February 9, [75] Effect of methylglyoxal on amyloid-beta folding links diabetes to Alzheimer s disease. RC Michelson, NW Seidler. Presented at the Sixteen Annual KCUMB Research Symposium, Kansas City, Missouri, February 9,

11 [76] Endogenous production of plastic composite material in AD brain: role of copper ions. NW Seidler, CK Harris. Presented at the 50 th International Conference AD/PD, Salzburg, Austria, March 14-18, Neurodegenerative Diseases (2007) 4(suppl 1):322, abstr [77] Production of neuromelanin from acrolein and serotonin in a two-phase liquid system of sevoflurane/water. RD Roberts, EE Fibuch, NW Seidler. Presented at Midwest Anesthesia Residents Conference 2007, St. Louis, Missouri, April 13-15, [78] Promotion of lipoxidation of human serum albumin by solvent hydrophobicty. HD Craig, NW Seidler. Presented at the 51 th Annual AOA Research Conference, San Diego, California, September 30-October 2, Published in J Am Osteopath Assoc (8):346, abstr. B01. [79] Effects of bilobalide on the conformation of Aβ. BR Odum, GJ Desai, NW Seidler, Presented at the 51 th Annual AOA Research Conference, San Diego, California, September 30-October 2, Published in J Am Osteopath Assoc (8):353, abstr. B20. [80] Novel mechanism of neurodegeneration: formation of polyacrolein in the presence of Cu(II) and albumin. PS Willsie, NW Seidler. Presented at the Sixteen Annual KCUMB Research Symposium, Kansas City, Missouri, February 8, [81] Isoflurane sequesters acrolein in the lipid phase of a two phase system. JE Johnson, EE Fibuch, NW Seidler. Presented at Midwest Anesthesia Residents Conference 2008, Indianapolis, Indiana, April 4-6, [82] Isoflurane modulates the selectivity of MGO modification of HSA. MR Baker, EE Fibuch, NW Seidler. Presented at Midwest Anesthesia Residents Conference 2008, Indianapolis, Indiana, April 4-6, [83] Isoflurane sequesters acrolein in the lipid phase of a two phase system. JE Johnson, EE Fibuch, NW Seidler. Presented at International Anesthesia Research Society (IARS) 82 nd Clinical and Scientific Congress, San Francisco, California, March 29-April 1, published in Anesthesia & Analgesia 2008; 106(3S):S-196. [84] Effects of methylglyoxal on amyloid-beta peptide. RC Michelson, NW Seidler. Presented at the Experimental Biology 2008, San Diego, California, April 8, Published in FASEB J 2008;22: [85] A proposed molecular action of sevoflurane on GAPDH. KT Cundiff, EE Fibuch, NW Seidler. Presented at Midwest Anesthesia Residents Conference 2009, Chicago, Illinois April 17-19, [86] Redox properties of serotonin-derived neuromelanin. MM Murphy, EE Fibuch, NW Seidler. Presented at Midwest Anesthesia Residents Conference 2009, Chicago, Illinois April 17-19, 2009 [87] Isoflurane modulates acetylation of albumin. SK Benton, EE Fibuch, NW Seidler. Presented at Midwest Anesthesia Residents Conference 2009, Chicago, Illinois April 17-19, 2009 [88] Computational analysis of shifts in the fluorescence spectra of human serum albumin. JD Eklund, NW Seidler. Presented at the 2009 International Conference on Bioinformatics and Computational Biology, Las Vegas, Nevada July 13-16, [89] Toxicity of a serotonin-derived neuromelanin. ED Miller, EE Fibuch, NW Seidler. Presented at Midwest Anesthesia Residents Conference 2010, Cincinnati, Ohio February 19-21, [90] Effects of isoflurane on plasma-membrane calcium ATPase. J Monaco, NW Seidler, EE Fibuch, A Zaidi, Presented at Midwest Anesthesia Residents Conference 2010, Cincinnati, Ohio February 19-21,

12 [91] Toxicity of a serotonin-derived neuromelanin. ED Miller, EE Fibuch, NW Seidler. Presented at Missouri Regional Life Sciences Summit, Kansas City, Missouri March , [92] Effects of isoflurane on the plasma-membrane calcium ATPase. J Monaco, NW Seidler, EE Fibuch, A Zaidi, Presented at Missouri Regional Life Sciences Summit, Kansas City, Missouri March , [93] Computational determination of aqueous concentrations of inhalational anesthetics. CS Theisen, NW Seidler. Presented at the 2010 International Conference on Bioinformatics and Computational Biology, Las Vegas, Nevada July 13-16, [94] The elderly brain: a chemical environment conducive to Alzheimer disease. RA High, CS Theisen, NW Seidler. Presented at the 54 th Annual AOA Research Conference, San Francisco, California, October 24-26, Published in J Am Osteopath Assoc 2010;110(8):469, abstr. B22. [89] Serotonin-derived melanoid from a sevoflurane interface is toxic to SH-SY5Y cells. TD Brownrigg, CS Theisen, EE Fibuch, NW Seidler. Presented at Midwest Anesthesia Residents Conference 2011, Chicago, Illinois April 1-3, [90] Isoflurane pre-conditioning involves upregulation of molecular chaperone genes. CA McClintick, CS Theisen, EE Fibuch, NW Seidler. Presented at Midwest Anesthesia Residents Conference 2011, Chicago, Illinois April 1-3, [91] Carnosine promotes GAPDH dimer formation: proposed role in preventing neurodegeneration. JY Kim, NW Seidler. Presented at the Experimental Biology 2011, Washington, D.C., April 10, Published in FASEB J 2011;25:572.6 [92] Solubility dynamics of amyloid-polyacrolein contribute to neurotoxicity. CS Theisen, NW Seidler. Presented at the Experimental Biology 2011, Washington, D.C., April 11, Published in FASEB J 2011;25: [93] Formation of a polyacrolein film at the water-anesthetic interface. RA High, NW Seidler. Presented at the Nineteen Annual KCUMB Research Symposium, Kansas City, Missouri, April 14, [94] Carnosine as a conformational switch for GAPDH. JY Kim, NW Seidler. Presented at the Nineteen Annual KCUMB Research Symposium, Kansas City, Missouri, April 14, [95] GAPDH architecture at low guanidine concentrations: first derivative analysis of the descending slope of the UV absorbance peak. JY Kim, CS Theisen, NW Seidler. Presented at the 2011 International Conference on Bioinformatics and Computational Biology, Las Vegas, Nevada July 18-21, [96] Protection against protein aggregation by alpha-crystallin as a mechanism of preconditioning. JE Ferns, CS Theisen, EE Fibuch, NW Seidler. Presented at Midwest Anesthesia Residents Conference 2012, Chicago, Illinois March 17, [97] Isoflurane s effect on the GABA A receptor mediated through GAPDH: a proposed model. SC McFarlane, R Anderson, CS Theisen, EE Fibuch, NW Seidler. Presented at Midwest Anesthesia Residents Conference 2012, Chicago, Illinois March 17, [98] Aβ-polyacrolein is neurotoxic in an amyloid-independent manner. CS Theisen, NW Seidler. Presented at the Experimental Biology 2012, San Diego, California, April 23, Published in FASEB J 2012;26:752.5 [99] Glyceraldehyde 3-phosphate dehydrogenase: classical example of moonlighting. NW Seidler. Presented at Science Friday Talks - Seminar Series. KCUMB, April 27, [100] Computational criteria for the disablement of human GAPDH pseudogenes. CS Theisen, KA Seidler, NW Seidler. Presented at the 2012 International Conference on Bioinformatics and Computational Biology, Las Vegas, Nevada July 16-19, [101] Proposed role of GAPDH pseudogene P44 in Alzheimer disease. SO Mason, NW Seidler. 12

13 Presented at the 8 th Leonard Berg Symposium on Prevention of Alzheimer s Disease. Washington University, Saint Louis, Missouri, September 28, [102] Isoflurane preconditioning involves the upregulation of aquaporin genes. ZK Schwamb, NW Seidler, CS Theisen. Presented at the Twenty-First Annual KCUMB Research Symposium, Kansas City, Missouri, November 1, [103] Neuronal protection by GAPDH pseudogene P44 variant. NW Seidler, SO Mason, CS Theisen. Presented at the 10 th Goettingen Meeting of the German Neuroscience Society. University of Goettingen, Goettingen, Germany, March 13, [104] Isoflurane preconditioning involves the upregulation of aquaporin genes. ZK Schwamb, NW Seidler, CS Theisen. Presented at the Experimental Biology 2013, Boston, Massachusetts, April 23, Published in FASEB J 2013;27:996.8 [105] GAPDH-mediated GABAA receptor trafficking enhanced by volatile anesthetics. AJ Montalbano, CS Theisen, EE Fibuch, NW Seidler. Presented at Midwest Anesthesia Residents Conference 2013, Kansas City, Missouri, April 27, [106] Isoflurane s effect on protein dynamics: implications on the mechanism of anesthesia. LA Roberts, EE Fibuch, NW Seidler. Presented at Midwest Anesthesia Residents Conference 2013, Kansas City, Missouri, April 27, [107]Effect of chloride ions on GAPDH conformation. OV Gorshkalova, NW Seidler. Presented at the 2013 International Conference on Bioinformatics and Computational Biology, Las Vegas, Nevada July 22-25,

14 Scholarly Activity Founded the Annual KCUMB Research Day Symposium (1993) Established the following courses at KCUMB: Discontinued COM course: Fundamental Principles of Cell and Molecular Biology (prior to the current Genesis Curriculum) Required COB course: BIOS-513 Biochemistry ( Present) Participated in Policy Construction: Sabbatical Policy (1995) Policy for Requesting Accommodations for Students with Disabilities (1997) Several policies for the KCUMB Research Committee ( ) Participated as Scientific Chairman: First Annual KCUMB Research Symposium, April 23, 1993 Second Annual KCUMB Research Symposium, April 1, 1994 Third Annual KCUMB Research Symposium, May 4, 1995 Fourth Annual KCUMB Research Symposium, May 10, 1996 Fifth Annual KCUMB Research Symposium, May 9, 1997 Sixth Annual KCUMB Research Symposium, February 27, 1998 Seventh Annual KCUMB Research Symposium, January 15, th Annual AOA Research Conference, Oct 26-Nov 1, 2000, Orlando, Florida 14

15 Committee Experience Kansas City University of Medicine and Biosciences Research Committee: : Member : Chairman Academic Accessibility Committee: : Member : Chairman Promotion and Graduation Committee: : Member : Chairman Institutional Review Board: : Member Rank and Promotion Committee: : Chairman Appeals Board: : Member Curriculum Committee: : Member Faculty Senate: : Executive Committee Member : President University of Central Missouri (Warrensburg, MO) Department of Biochemistry, Chemistry and Physics Advisory Board 2008 Peer Review Journal Reviewer Amino Acids (2011); BBA-Molecular Basis of Disease (2004, 2005); BBA-General Subjects (2006); Biochemical Journal (2011); Biochemistry (2006, 2007); Journal of Pharmacy and Pharmacology (2013); FEBS Letters (2007); Molecular and Cellular Biochemistry (2011, 2006); Neurochemical Research (2009); The Protein Journal (2010) Editorial Board Member Journal of Anesthesia and Clinical Research (2010-present); Advances in Anesthesiology (2013- present) Grant Reviewer Alzheimer s Association (2007-present) 15

16 Curricular Experience KCUMB-College of Osteopathic Medicine, Section Director: : MED-101 Foundations of Medicine : MED-210 Skin, Blood and Lymph KCUMB-College of Biosciences, Course Director: : BIOS-513 Biochemistry Memberships in professional organizations American Society for Biochemistry and Molecular Biology Biophysical Society Protein Society International Maillard Reaction Society Honors and awards Dr. Seidler May 1981: May 1994: April 2000: January 2003: December 2004: January 2004: February 2005: February 2006: Awarded honorary membership in Sigma Xi Research Society Instructor of the Year 1994, KCUMB Faculty Research Publication Award, KCUMB Outstanding Faculty Oral Research Presentation Award 11th Annual KCUMB Research Symposium Governor s Excellence in Teaching Award Governor Bob Holden, Springfield, Missouri Outstanding Faculty Oral Research Presentation Award 12th Annual KCUMB Research Symposium Outstanding Faculty Oral Research Presentation Award 13th Annual KCUMB Research Symposium Outstanding Faculty Oral Research Presentation Award 14th Annual KCUMB Research Symposium 16

17 Honors and awards Dr. Seidler NIH National Research Service Award, National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMSD), AR , September 1989-June 1991 Beginning Grant-In-Aid, American Heart Association, Kansas Affiliate, KS-95-GB-13, July 1995-January 1997 Intramural Grant, Division of Research, KCUMB Regulation of proteolysis of the cardiac aspartate aminotransferase (2000) Intramural Grant, Division of Research, KCUMB Anti-glycation effects of histidine (2001) Intramural Grant, Division of Research, KCUMB Mechanism of cataract reversal by carnosine (2002) Intramural Grant, Division of Research, KCUMB Histidinyl-compounds as small molecular weight chaperones: novel therapeutic agents for protein misfolding diseases (2003) Intramural Grant, Division of Research, KCUMB Tracking Aβ fibrillogenesis using tyrosine fluorescence ( ) Intramural Funding, College of Biosciences, KCUMB Effects of methylglyoxal on the properties of amyloid-beta; Randal C. Michelson ( ) 17

18 Honors and awards - Dr. Seidler s research trainees (students and residents) Basic Science Research Award: (KCUMB College of Osteopathic Medicine) Shailesh U. Rajguru, Class of 1995 Paul C. Guichard, Class of 1996 William Snider, Class of 1998 Michael Cassetta, Class of 1999 Catherine Fitzgerald, Class of 2000 Timothy A. Swearengin, Class of 2001 Justin Nauth, Class of 2002 Sharagim S. Shokry, Class of 2002 James L. Southwell, Class of 2003 Catherine Kowalewski, Class of 2004 Trevor J. Squire, Class of 2008 Caitlin Harris, Class of 2009 Clinical Research Award: (KCUMB - College of Osteopathic Medicine) Brad Cucchetti, Class of 2001 Laura Hobart, Class of 2005 Outstanding Student Oral Research Presentation: (Annual KCUMB Research Symposium) Shailesh U. Rajguru, Class of 1995 (1st Place) Paul C. Guichard, Class of 1996 (1st Place) William Snider, Class of 1998 (1st Place) Justin Nauth, Class of 2002 (1st Place) Sharagim S. Shokry, Class of 2002 (3rd Place) Laura J. Hobart, Class of 2005 (2nd Place) Tim G. Morgan, Class of 2006 (3rd Place) Ankur Mehta, Class of 2007 (1st Place) Outstanding Student Research Poster Presentation: (Annual KCUMB Research Symposium) Trevor J. Squire, Class of 2008 (1st Place) Jessica Kim, Class of 2013 (3 rd Place) Rachel A. High, Class of 2013 (3 rd Place) 1993 Burnett Osteopathic Student Research Award: (AOA - Bureau of Research) Joseph E. Burkhardt, Class of Medical Student Research Competition: (American College of Physicians, Philadelphia, PA) Farhad Farokhi, Class of 1996 (Finalist) 18

19 2001 AOA Outstanding Student Research Poster Presentation: (AOA - Bureau of Research) James L. Southwell, Class of 2003 Outstanding Resident Research Presentation: (Annual Hospital Staff Research Day, Saint Lukes Hospital) Ryan D. Roberts, MD (2007; 2 nd Place) Michelle R. Baker, PhD, MD (2008; 1 st Place) Andrew Montalbano, MD (2013; 1 st Place) (Annual Midwest Anesthesia Residents Conference) Meghan Murphy, DO (2009, Chicago, IL; 1 st Place, Neuroanesthesia Section) Tanner D. Brownrigg, MD (2011, Chicago, IL; 1 st Place, Clinical Pharmacology Section) Chad A. McClintick, DO (2011, Chicago, IL; 2 nd Place, Clinical Pharmacology Section) Jonathan E. Ferns, MD (2012, Chicago, IL; 3 rd Place, Clinical Pharmacology Section) Sally C. McFarlane, MD (2012, Chicago, IL; 1 st Place, Clinical Pharmacology Section) 19