Meeting Report. Working Group 2 (WG2) Strategies for Autophagy analyses and modulation

Transcription

1 Meeting Report Working Group 2 (WG2) Strategies for Autophagy analyses and modulation March 2017 Interfaculty Institute of Cell Biology Eberhard Karls University Tübingen Auf der Morgenstelle 15 D Tübingen Germany Organizer Tassula Proikas-Cezanne Frank Lafont 1

2 Announcement goce risteski/fotolia.de The History of Autophagy COST Action TRANSAUTOPHAGY Working Group 2 (WG2) Meeting Tübingen Workshop March 2017 Interfaculty Institute of Cell Biology, Eberhard Karls University Tübingen, Auf der Morgenstelle 15, Tübingen, Germany Speakers KEYNOTE: Nektarios Tavernarakis Foundation for Research and Technology Hellas Heraklion, Crete, Greece Frank Lafont CNRS (UMR8204), Pasteur Institute of Lille, Lille, France Antonio Zorzano University of Barcelona, IRB, CIBERDEM, Barcelona, Spain Guiseppe Maulucci Università Cattolica del Sacro Cuore, Milano, Italy Antonio Cuadrado Universidad Autónoma de Madrid, Madrid, Spain Nikolai Engedal University of Oslo, Oslo, Norway Mario Tschan Institute of Pathology, University of Bern, Bern, Switzerland Tassula Proikas-Cezanne Eberhard Karls University Tübingen, Germany Strategies for Autophagy analyses and modulation Public communication Academic publications Training School Translational potential +++ Talks +++ Discussion +++ Brainstorming +++ Planning +++ Organizers Tassula Proikas-Cezanne (WG2 speaker) Frank Lafont (WG2 Co-speaker) Contact tassula.proikas-cezanne@uni-tuebingen.de 2

3 Schedule COST Action TRANSAUTOPHAGY Working Group 2 (WG2) Meeting March 2017, Eberhard Karls University Tübingen Monday, :45 h From 18:00 h Travel Airplane to Stuttgart Airport or Train to Train Station Tübingen Hotel Check In from 15:00 h Transport from Stuttgart Airport (STR) to Meeting location Please meet Daniel Reitter at the Meeting Point Registration Rooms booked for you at: Hotel Krone, Uhlandstraße 1, Tübingen Phone: Interfaculty Institute of Cell Biology, Eberhard Karls University Tübingen Auf der Morgenstelle Tübingen, Germany 2. Floor, Foyer 18:30 h Opening Seminar room :00 h Welcome from the organizer Frank Lafont (Co-Speaker) 19:10 h WG2: Aims, Plans, Actions Tassula Proikas-Cezanne (Speaker) 19:20 h Discussion Moderator: James Murray 19:30 h Chair: Keynote lecture Foundation for Research and Technology - Hellas Frank Nektarios Tavernarakis Heraklion, Crete, Greece Lafont 20:15 h Discussion 20:30 h Buffet 2. Floor, Foyer Available: Transport to Hotel Krone 3

4 Tuesday, :30 h Chair: James Murray Seminar room opens at 08:00 h Frank Lafont Subversion of autophagy by pathogens studied using correlative nanoscopy approaches 09:00 h Discussion 09:15 h Antonio Zorzano Chair: A novel mechanism of regulation of adiposity Monika Cahova based on the autophagy protein TP53INP2 09:45 h Discussion 10:00 h Coffee break 10:30 h Chair: Soren Hayrabedyan 11:00 h Discussion 11:15 h Chair: Soren Hayrabedyan 11:45 h Discussion 12:00 h Lunch buffet Guiseppe Maulucci Quantitative analysis of autophagic flux by confocal ph-imaging of autophagic intermediates Antonio Cuadrado Regulation of macroautophagy and chapareone mediated autophagy by transcription factor NRF2 13:00 h Nikolai Engedal Chair: Advantages of using cargo-based assays Krassimira instead of LC3 to monitor autophagic activity and Todorova its regulation 13:30 h Discussion 13:45 h Mario Tschan Chair: Assessing autophagy in primary tumor tissue James Murray 14:15 h Discussion 14:30 h Tassula Proikas-Cezanne Chair: Antonio Using WIPI proteins for autophagy analysis and Zorzano modulation 15:00 h Discussion Interfaculty Institute of Cell Biology Eberhard Karls University Tübingen Auf der Morgenstelle Floor, Seminar room CNRS (UMR8204) Pasteur Institute of Lille, Lille, France University of Barcelona, IRB, CIBERDEM, Barcelona, Spain 1. Floor, Foyer Poster presentation Università Cattolica del Sacro Cuore, Milano, Italy Universidad Autónoma de Madrid, Madrid, Spain 1. Floor, Foyer Poster presentation University of Oslo, Oslo, Norway Institute of Pathology, University of Bern, Bern, Switzerland Eberhard Karls University Tübingen, Germany 15:15 h Coffee break 1. Floor, Foyer Poster presentation 15:45 h Discussion WG2: Aims, Plans, Actions Moderator: Nektarios Tavernarakis 17:30 h Transport to Hotel Krone 18:30 h Sightseeing Tour Tübingen Meeting Point: Hotel Krone 19:30 h Dinner (Walking distance to Hotel Krone) Neckarmüller, Restaurant, Brewery, Gartenstr. 4, Tübingen 4

5 Wednesday, :15 h 08:30 h 09:00 h 09:15 h 10:15 h Seminar room opens at 08:00 h Frank Lafont Summary of discussions: WG2: Aims, Plans, Actions Discussion Moderator: Nikolai Engedal Tassula Proikas-Cezanne Next Steps: WG2: Aims, Plans, Actions Discussion Moderator: Mario Tschan Voting: WG2: Aims, Plans, Actions Moderator: Nikolai Engedal Eberhard Karls University Tübingen, Interfaculty Institute of Cell Biology Auf der Morgenstelle Floor, Seminar room :30 h Coffee break 1. Floor, Foyer 10:45 h Tassula & Frank Meeting summary 10:50 h End of Meeting Lunch Packet 11:00 h Available: Transport to Stuttgart Airport 5

6 Participants Statistics Participants: 27 Countries: 11 Female scientists: 14 (3 senior*, 11 junior female scientists) Male scientists: 13 (9 senior*, 4 junior male scientists) * senior scientist defined as holding at least a PhD 6

7 Meeting report WG2 deliverables 1. Summarize procedures for stage-specific assessments of autophagy capacity and activity 2. Build up open-access software concepts for autophagy-related data mining in systems biology approaches 3. Advice on procedures for chemical and biological screening for novel, clinically-relevant autophagy modulators 4. Promote concepts for company start-ups and coordinate experimental cooperation between industry and academia 5. Suggestions for the employment of potential technologies, including nanotechnologies, to artificially modulate autophagy in intended biomedical and biotechnological purposes The first WG2 meeting fulfilled the following requirements to meet the WG2 deliverables (as listed above): Discussing the different autophagy research areas of the participants (talks and subsequent discussions) Discussing different autophagy assessments (talks and discussions) Discussing deliverable (see above, WG2 deliverable 1., 3., 4., 5) Planning deliverables (see above, WG2 deliverable 1., 3., 4., 5) Discussing and planning Training School The participants agreed to the following actions with regard to WG2 deliverables 1. Summarize procedures for stage-specific assessments of autophagy capacity and activity and 3. Advice on procedures for chemical and biological screening for novel, clinically-relevant autophagy modulators : Organise the review writing for the special issue featuring Transautophagy in the journal Oxidative Medicine and Cellular Longevity (Coordinator: Engedal, Cahova) - Inquire extension of deadline (Engedal) - Prepare table of content and list contributors (deadline 1 st May 2017) 7

8 - Contact WG1 to coordinate review writing with regard to Essays in Biochemistry and Frontiers in Oncology Special Issue on: TRANSAUTOPHAGY: Research and Translation of Autophagy Knowledge. Lead Guest Editor Maria Cristina Albertini University of Urbino Carlo Bo, Urbino, Italy. Guest Editors Tassula Proikas-Cezanne Eberhard Karls University Tübingen, Tübingen, Germany. Nikolai Engedal Oslo University, Oslo, Norway. Eva Žerovnik Institut Jozef Stefan, Ljubljana, Slovenia. Contact the Journal of Pathology to inquire the possibility to submit an extensive review on the WG2 deliverables 1./3./5. with open access (Coordinator: Tschan, Cuadrado) - Topics: Opportunities and pitfalls in interpretation of clinical autophagy assessment (e.g. stress response versus autophagy) - Prepare table of content and list contributors (deadline 1 st May 2017) - Contact WG1 to coordinate review writing with regard to Essays in Biochemistry and Frontiers in Oncology Contact the journals Autophagy and Journal of Cell Science to inquire the possibility to submit an extensive review on the WG2 deliverables 1./3./5. with open access (Coordinator: Lafont, Proikas-Cezanne) - Topics: Summarizing HTP efforts in autophagy assessments and discussing the different methods in detail with listing limitations in autophagy flux assays (e.g. using LC3 as a marker) - Prepare table of content and list contributors (deadline 1 st May 2017) - Contact WG1 to coordinate review writing with regard to Essays in Biochemistry and Frontiers in Oncology - Contact cost members with regard to experiences in HTP assays including bioinformatics and system biology 8

9 The participants agreed to ask Cost Action Transautophagy to support open access publishing with 2000,- for each review, whereby the compete sum will be financed by the contributing co-authors (Coordinator: Proikas-Cezanne, Lafont) The participants agreed to prepare an open access video animation on autophagy for educational scientific and public communication. For this purpose, preliminary discussions have been initiated with CAMPUS TV at the University of Tübingen (Proikas-Cezanne). The project should be operated by young members of the German autophagy network (GBM Studiengruppe Autophagie) with CAMPUS TV having concept and scheme circulating among WG2 participant for final approval. Ask Cost Action Transautophagy for financial support (2500,- ) (Coordinator Proikas-Cezanne). With regard to the WG2 deliverable 4 Promote concepts for company start-ups and coordinate experimental cooperation between industry and academia the participants discussed the possibility to start a spin-off (SO) company on autophagy assessments as we (WG2) have the expertise, the assays and the technology in our labs and institutional facilities. We (WG2) may propose to develop a SO to conduct assays for academics and industries. In this context the following questions where raised and discussed: - pro and contras of having a formal office and laboratories in addition to the participating laboratories of the individual PIs - business plan - funds for instruments - fund raising opportunities within EU-H2020 and/or national systems - fund raising opportunities through companies for testing reagents and procedures - crowdfunding - patents - databases - consultancies - short-term mission: define aims, participants, expertise - mid-term mission: business plan - summary on aims, participants, expertise by 1 st June Coordinator Proikas-Cezanne 9

10 The participants discussed planning of the TRAINING SCHOOL (TS) - participants agreed that the financial situation is not satisfactorily as the allowance so far is below the minimum to organize the TS - discuss the need for a substantial increase - alternatives: refine the solution proposed with the offer in Lille, use short mission budget for the practical, increase the theoretical part, open call for participants outside the Cost Action Transautophagy (registration fees), conduct the TS in Brussels or Alicante - Coordinator Lafont The participants agreed to support an international conference of the German Society for Biochemistry and Molecular Biology (GBM) organized by the German Autophagy Network (GBM Studiengruppe Autophagie) on the topic of Autophagy and Aging in 2019 in Tübingen, DE. The participants agreed that the next WG2 Meeting will be held in March 2018 in Sofia, Bulgaria. Meeting Summary The participants agreed to - write 3 review articles (2017) - prepare a video animation for scientific educational and public communication (2017) - further discuss the common interest to found a spin-off company (2017) - refine costs, topics and location of the TS (2018) if required - prepare the 2. WG2 Meeting in Sofia/BG (2018) - support the International GBM Conference Autophagy and Aging in Tübingen, DE (2019) 10